包军;张名岳;李想;刘洪贵;李剑虹;高利;张晓卉;王博;

• 1:东北农业大学动物科学技术学院
• 2:东北农业大学生命科学学院
• 3:东北农业大学动物医学学院
• 4:哈尔滨工业大学校医院

摘要(Abstract)：

为研究母猪脑海马死亡后变化情况,试验以同一遗传背景(杜×长×大)、体重及出生日龄相近的健康雌性仔猪作研究对象,采用核磁共振成像检测及光镜技术,检测母猪麻醉状态与死后10 min、1、2、3、6和10 h时段左侧、右侧海马体积,观察两侧海马齿状回区颗粒细胞形态,建立母猪死后海马形态学变化模型,为长期处于应激状态下妊娠母猪心理疾病的核磁共振诊断提供理论依据。核磁检测结果表明,仔猪死亡10 h内,与活体对照组海马体积比较,左侧海马体积无显著变化(P=0.901);右侧及总海马体积在死亡3 h内无显著性变化(右侧P=0.078;总P=0.104),但从死亡6 h开始显著变小(右侧P<0.01;总P<0.05);光镜观察表明,仔猪死亡3 h内,左侧及右侧海马齿状回区颗粒细胞形态与活体对照组相比,均未发生严重损害。死亡3 h内,仔猪脑海马组织无显著生理变化。

关键词(KeyWords)： 核磁共振成像;雌性仔猪;海马体积;颗粒细胞;死亡时间

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31472131/C170109)

作者(Author): 包军;张名岳;李想;刘洪贵;李剑虹;高利;张晓卉;王博;

Email:

参考文献(References)：

• [1]Schouten W G,Wiepkema P R.Coping styles of tethered sows[J].Behavioural Processes,1991,25(2-3):125-132.
• [2]Marchant J N,Broom D M.Factors affecting posture changing in loose-housed and confined gestating sows[J].Animal Science,1996,63(3),477-485.
• [3]Averós X,Brossard L,Dourmad J Y,et al.Quantitative assessment of the effects of space allowance,group size and floor characteristics on the lying behaviour of growing-finishing pigs[J].Animal,2010,4(5):777-783.
• [4]Antonijevic I A.Depressive disorders-is it time to endorse different pathophysiologies[J].Psychoneuroendocrinology,2006,31(1):1-15.
• [5]Frazer A,Morilak D A.What should animal models of depression model[J].Neuroscience Biobehavioral Review,2005,29(4-5):515-523.
• [6]Broom D M.Indicators of poor welfare[J].British Veterinary Journal,1986,42:438-439.
• [7]Broom D M.Relationship between welfare&disease susceptibility in farm animals[C]//Bva Animal Welfare Foundations,1987.
• [8]Van der Staay F J,Schuurman T,Hulst M,et al.Effects of chronic stress:A comparison between tethered and loose sows[J].Physiology Behavior,2010,100(2):154-164.
• [9]Olff M.Stress,depression and immunity:the role of defense and coping styles.[J].Psychiatry research,1999,85(1):7-15.
• [10]Ehlert U,Gaab J,Heinrichs M.Psychoneuroendocrinological contributions to the etiology of depression,posttraumatic stress disorder,and stress-related bodily disorders:the role of the hypothalamus-pituitary-adrenal axis[J].Biological Psychology,2001,57(1-3):141.
• [11]Mc Kittrick C R,Magarinos A M,Blanchard D C,et al.Chronic social stress reduces dendritic arbors in CA3 hippocampus and decreases binding to serotonin transporter sites[J].Synapse,2000,36(2):85-94.
• [12]Mc Laughlin K J,Baran S E,Wright R L,et al.Chronic stress enhances spatial memory in ovariectomized female rats despite CA3 dendritic retraction:possible involvement of CA1 neurons[J].Neuroscience,2005,135(4):1045-1054.
• [13]Drevets W C,Price J L,Furey M L.Brain structural and functional abnormalities in mood disorders:implications for neurocircuitry models of depression[J].Brain Structure and Function,2008,213(1):93-118.
• [14]Lv F L,Li X,Li J H,et al.Morphological changes in hippocampus and prefrontal cortex of confined sows with prolonged pupillary light reflex[J].Journal of Northeast Agricultural UniversityEnglish Edition,2016,23(3):39-46.
• [15]Sheline Y I.Depression and the Hippocampus:Cause or Effect?[J]Biological Psychiatry,2011,70(4):308-309.
• [16]安志远,张晓东,郑吉龙,等.脑组织与法医学死亡时间推断[J].中国法医学杂志,2009,24(4):257-259.
• [17]Lind N M,Moustgaard A,Jelsing J,et al.The use of pigs in neuroscience:modeling brain disorders[J].Neuroscience&Biobehavioral Reviews,2007,31(5):728-751.
• [18]Gy?rffy-Wagner Z,Englund E,Larsson E M,et al.Proton magnetic resonance relaxation times T1 and T2 related to postmortem interval.An investigation on porcine brain tissue[J].Acta Radiologica Diagnosis,1986,27(1):115-118.
• [19]Holm I E,West M J.Hippocampus of the Domestic Pig:A stereological study of subdivisional volumes and neuron numbers[J].Hippocampus,1994,4(1):115-126.
• [20]Amunts K,Kedo O,Kindler M,et al.Cytoarchitectonic mapping of the human amygdala,hippocampal region and entorhinal cortex:intersubject variability and probability maps[J].Brain Structure and Function,2005,210(5):343-352.
• [21]Ryu S Y,Kwon M J,Lee S B,et al.Measurement of Precuneal and Hippocampal Volumes Using Magnetic Resonance Volumetry in Alzheimer's Disease[J].Journal of Clinical Neurology,2010,6(4):196-203.
• [22]Hatipoglu H G,Ozcan H N,Hatipoglu U S,et al.Age,sex and body mass index in relation to calvarial diploe thickness and craniometric data on MRI[J].Forensic Science International,2008,182(1-3):46-51.
• [23]Bartsch T,Wulff P.The hippocampus in aging and disease:From plasticity to vulnerability.[J].Neuroscience,2015,309:1-16.
• [24]Falconer M A,Serafetinides E A,Corsellis J A.Etiology andpathogenesis of temporal lobe epilepsy[J].Archives of Neurology,1964,10(8):233-248.
• [25]Ashtari M,Barr W B,Schaul N,et al.Three-dimensional fast low-angle shot imaging and computerized volume measurement of the hippocampus in patients with chronic epilepsy of the temporal lobe[J].American Journal of Neuroradiology,1991,12(5):941-947.
• [26]Jelsing J,Rostrup E,Markenroth K,et al.Assessment of in vivo MR imaging compared to physical sections in vitro-a quantitative study of brain volumes using stereology[J].Neuroimage,2005,26(1):57-65.